Molecular Characterization of Carbapenem-Resistant <i>Acinetobacter baumannii</i> Blood Culture Isolates from Three Hospitals in Turkey

We aimed to investigate the clonal relationships, common sequence types, and carbapenemase genes in 177 non-repetitive blood culture isolates of Acinetobacter baumannii collected from patients at three university hospitals Turkey 2016. Molecular epidemiological characteristics were examined using pulsed-field gel electrophoresis (PFGE), multilocus typing (MLST) (Pasteur scheme-cpn60, fusA, gltA, pyrG, recA, rplB, rpoB). Multiplex PCR was used genes, including blaOXA-23-like, blaOXA-24-like, blaOXA-48-like, blaOXA-58-like, blaIMP, blaVIM, blaNDM. PFGE genotyping yielded 92 pulsotypes with a clustering ratio 69.7%. As per ?85% similarity coefficient, 159 (90.9%) found be clonally related. The blaOXA-23-like blaOXA-58-like identified 100% 28.2% isolates, respectively. blaNDM gene two isolates. MLST analysis included 54 different pulsotypes, 29 types (STs). Most (n = 36) belonged complex (CC)2, one isolate CC1, CC164. Sixteen new STs (ST1235–ST1250) identified. Identifying both global ST2 large number STs, revealed high genetic diversity A. study population.